HYBRID EVENT: You can participate in person at Valencia, Spain or Virtually from your home or work.
Hybrid Event
September 18-20, 2023 | Valencia, Spain
ICTM 2022

Lu Zhang

Lu Zhang , Speaker at Traditional Medicine Conference 2022
The University of Hong Kong, Hong Kong
Title : Targeting the activation of Microglia and M2-like shift in Multiple Sclerosis: from Mechanisms Study to Therapeutic intervention with Ganoderma Lucidum Spore Powder

Abstract:

Objective:

To investigate whether the Ganoderma Lucidum Spore (GLS) powder could alleviate the experimental autoimmune encephalomyelitis by attenuating the activation and polarization of microglia both in vivo and vitro.

Methods:

C57BL/6N female mice (~10 weeks, n=7     for each group) were induced by CFA and MOG35-55 with intravenous injection of Pertussis Toxin. Two treatment protocols were adopted: for pretreatment protocol, the GLS was orally administrated from day 2 to day 30 post-immunization, for the early prevention protocol, GLS was orally administrated 7 days before immunization until day 30 post-immunization. Daily assessment of body weight and clinical score were processed. Hematoxylin & Eosin (H&E), Luxol Fast Blue(LFB), Immunohistochemical (IHC) and immunofluorescence (IF) methods were applied to determine the inflammatory infiltration, demyelination, activation and polarization of microglia in mouse central nervous system. The Western Blot and qPCR were applied to elucidate the potential mechanism.

Result:

The result revealed that the GLP could alleviate experimental autoimmune encephalomyelitis (EAE) progression, delay the onset of EAE and reduce the severity of EAE. For the H&E and LFB results, GLP was indicated to reduce the inflammatory infiltration and demyelination in the spinal cord of EAE immunized mice (**p<0.01). The IHC and IF results indicated the activation of microglia and the M1/M2 ratio of microglia was significantly reduced (P<0.05). The inflammatory related cytokines such as IL-4, and IL-10 was reduced after GLS treatment. Besides, GLS was revealed to attenuate the microglial apoptosis, phagocytosis, and free radical generation to have beneficial effects.

Conclusions:

Overall, the GLP treatment could alleviate the EAE to indicate a potential therapeutic approach toward Multiple Sclerosis. GLP revealed to have a positive impact on immune modulation, neuroprotection, anti-apoptosis, and anti-oxidation via mediating the activation and polarization of microglia through attenuating NF-kB pathway.

Biography:

ZHANG Lu studied Biological Chemistry at the Sun Yat-sen University, China and graduated as Bachelor at 2016. Then she graduated as Master of Analytical Chemistry in 2017 in Hong Kong Baptist University. She then joined the research group of Prof. Jiangang SHEN at the School of Chinese Medicine, the University of Hong Kong since 2018, and then admitted as Ph.D. student since 2019 with the research area of free radicals and immune modulation in central nervous system.

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