Dose variant anti-diabetic properties of Zanthoxylum armatum DC. Fruits on n-STZ induced Type-2 Diabetic Model rats and Evaluation of toxicity

Title : Dose variant anti-diabetic properties of Zanthoxylum armatum DC. Fruits on n-STZ induced Type-2 Diabetic Model rats and Evaluation of toxicity

Abstract:

Zanthoxylum armatum is one of the highly valued medicinal plant used for treatment of different ailments including diabetes. This study aimed to screen anti-diabetic effect of methanolic extract of fruits of Zanthoxylum armatum(ZAFME) on neonatal streptozotocin (nSTZ) induced Type II diabetic (T2DM) Rats and to find out the extract toxicity on normal rats and mice. STZ was administered in 48hrs pups by a single ip injection and after three months OGTT was done. Rats were divided into five groups: i) Normal control, ii) Diabetic control; iii) Positive control and Extract treated groups iv & v. Experimental period of 28 days on groups i& ii received water, groups iii, iv & v received  Gliclazide and 25mg and 50mg/kg bw of ZAFME with a single feeding respectively. Blood samples collection by tail cut at the beginning and by cardiac puncture at the end of the experiment was done for fasting serum glucose. Statistical analysis was done by one-way ANOVA and paired sample t-test. For toxicity testing, single-dose oral feeding was done in geometrical ratio from 200mg/kgbd.wt to 3200mg/kg bdwt. Lorke method was used for toxicity determination. There were significantly lower blood glucose values in both groups of  ZAFME and gliclazide group at Endpoint compared to Baseline (p<0.041, p<0.023 and 0.003 respectively). Moreover, at the end point, ZAFME at 50mg dose decreased blood glucose significantly (p,0.021) compared to Diabetic control. In the Acute toxicity test there were no significant changes in Long Evans rats whereas in Swiss Albino mice LD₅₀ value was 565.68 mg. Histopathological observation of Swiss Albino mice revealed normal liver, pancreas, and kidney at dose level 200 mg whereas higher dose has inflammatory changes, polymorphs, degenerative changes and necrosis in liver, pancreas, and kidney respectively. These findings highlight the therapeutic potential of the extracts and provide strong impetus for further studies with the safer dose of 565.68 mg in the case of Swiss albino mice.

Biography:

Mrs. Janaki Baral. She served as lecturer at Little Angels’ Higher Secondary School (2010-2017) Lalitpur after completing her masters. She is serving nation as an assistant Professor in one of the oldest and largest University of Nepal: Tribhuvan University, Nepal since 2017. Her MSc is in organic chemistry with interest on the research areas of isolation of compounds from natural products, in-vivo and in-vitro determination of antidiabetic property, toxicity determination, anti-cancerous activity determination of plant extracts, fractions and compounds, and other biological activity determination. She had completed her M.Phil. in Educational Leadership from Kathmandu University. She then joined the group of Associate Professor Dr. Achyut Adhikari for her PhD at Tribhuvan University. She was an ANRAP fellow (2018), at Bangladesh University of Health Sciences, Bangladesh. She had also visited University of Pakistan for her research activity. She is co-author of 2 articles, national and international. Active involvement in social activities.