HYBRID EVENT: You can participate in person at Madrid, Spain or Virtually from your home or work.
Madrid, Spain
September 05-07, 2024
ICTM 2024

Emmanuel Friday Titus

Emmanuel Friday Titus, Speaker at Traditional Medicine Conferences
Prince Abubakar Audu University Anyigba Nigeria, Nigeria
Title : Phyto-constitution of methanolic leaf extract of Ficus capensis and its biochemical, haematological, and toxicity effects on wistar rats


Statement of the Problem: Ficus capensis belongs to the Moraceae family and has been reported to possess various pharmacological properties. However, it is crucial to establish the toxicological profile of this important medicinal plant. Thus, this study investigates the phytoconstituents of methanolic leaf extract of Ficus capensis, and its biochemical, haematological, and toxicity effects using the Wistar rat model. Methodology: Twenty Wistar rats were obtained and then divided into four groups; Group 1 (control) received distilled water, while Groups 2, 3 and 4 orally received the graded doses (10, 100, and 1000 mg/kg, respectively) of the leaf extract for twenty-eight (28) days. All the animals were sacrificed after 28 days of treatment. The phytochemicals, proximate analysis, acute and sub-chronic toxicity were determined on crude leaf extract using standard procedures. Genotoxicity was determined by Comet Assay, biochemical assays (alanine transaminase, aspartate transaminase, alkaline phosphatase, total protein and total bilirubin) were performed using Randox kits, and haematological parameters were determined using automated haematology analyser. Findings: Phytochemical analysis showed the presence of tannin (21.47±0.01 mg), total phenol (148.89±0.04 mg), flavonoid (67.00±0.06 mg), alkaloid (1.06±0.00%), saponin (62.94±0.05 mg) and cardiac glycosides (4.60±0.00 mg) while proximate analysis showed 11.66% moisture, 13.50% ash, 7.80% lipid, 19.69% protein, 10.20% crude fibre, and 37.15% carbohydrates. No mortality or apparent signs of toxicity (up to 5000 mg/kg) was shown. The extract had no significant effect (p>0.05) on the biochemical and haematological parameters and the histology of the liver and kidney. The comet assay revealed no genetic damage at all doses administered, although the % tail DNA and tail moment at 100 and 1000 mg/kg were significantly different (p<0.05) from the control. Conclusion & Significance: Leaf extract of Ficus capensis contains bioactive compounds and no form of toxicity and can be used as an alternative source of therapy.


Dr Emmanuel Friday Titus is a senior lecturer at the Prince Abubakar Audu University with more than two decades of teaching and research experience. During this period, he has taught different category of undergraduate and graduate students in the field of biochemistry and toxicology, and has mentored the students in various research projects. He is a passionate scientist majoring in research that are focused on biochemical, environmental, nutritional, and phyto-toxicity using various in vitro and in vivo experimental models. He is also passionate about the mechanism through which medicinal plants could ameliorate diseases in a safe and reliable manner. The impact of his research has been demonstrated by scholarly publications in national and international journals.