Isolation of bioflavanoid from beet root and evaluation of antitumour actvity followed by molecular docking against topoisomerase-I

Title : Isolation of bioflavanoid from beet root and evaluation of antitumour actvity followed by molecular docking against topoisomerase-I

Abstract:

The main aim and objective of my present research work was to screen the primary and secondary metabolites and isolate the bioactive flavanoid vitexin and isovitexin present in the methanolic extract of beet root (Beta vulgaris) and evaluation of antitumour activity. The isolation of bioflavonoids were done by U. V spectrophotometer; ABB Bomem FT-IR spectrometer; Bruker NMR spectrometer; HPLC-MS etc. The moleculr docking of isolated vitexin was carried out against target protein 1A36-Topoisomerase I by using Auto-dock programme. Evaluation of in vivo antitumour activity of ME-BRT against Sarcoma-180 Ascite (S-180-AC) in rat models. In silico molecular docking studies displayed the binding energies: -3.95 k.cal/mol, of vitexin which indicated that the compound had high binding affinity towards the 1A36 -Topoisomerase I in comparison with std. drug topotecan (-2.06 k.cal/mol). From the present experimental data here we concluded that the Methanolic Extracts of Beet Root (ME-BRT) showed the potential ability to inhibit the growth of tumour induced by SARCOMA-180 ascites in mouse model and in silico molecular docking results displayed that vitexin had the higher affinity to bind with topoisomerase-I enzyme in comparison to standard drug topotecan and act as potential inhibitor of topoisomerase-I.
Keywords: Vitexin, Antitumour Activity, Sarcoma, Molecular Docking etc.

Audience Take Away Notes

• Research in natural product chemistry, such as isolating biomolecules and evaluating their pharmacological effects, including antitumor activity, provides valuable insights for drug discovery.
• By analyzing compounds from medicinal plants, scientists integrate traditional knowledge with modern science to develop new therapeutic agents.
• This approach not only validates and refines traditional remedies through rigorous scientific methods but also harnesses these natural substances to create innovative drugs.
• The synergy between historical wisdom and cutting-edge research enables the exploration of novel treatments, making natural product chemistry crucial for advancing pharmaceutical development and enhancing health outcomes.

Biography:

Dr. Asish Bhaumik, M. Pharm; PGDAIB; PGDCR; PhD, is an Associate Professor in Pharmaceutical Chemistry at School of Pharmaceutical Sciences, Girijananda Chowdhury University, Tezpur, Assam. He completed his B. Pharm at RIPSAT, Agartala, and his M. Pharm at C. L. Baid Metha College, Chennai. His postgraduate studies include Diplomas in Applied and Industrial Biotechnology and Clinical Research. His PhD, from Assam Downtown University, focused on Pharmacology, Biochemistry, and Medicinal Chemistry. Dr. Bhaumik teaches and researches in these fields, with 75 publications and 60 conference presentations. He actively participates in academic events, serves on the Doctoral Committee, and supervises PhD scholars.