Title : Effects of leave-extract of some medical plants on biochemical and reproductive parameters in rat fed high-salt diet
Abstract:
There is increase in prevalence of people living with various forms of human diseases such as reproductive problems. The causes and maintenance of these problems is limited in the literature, although diet has been implicated. Due to high cost of assessing basic standard medical fertilities, people in the local communities of Africa nation have resulted to the use of herbal mixtures as an alternative form of therapy without having detailed knowledge of its efficacy and possible side effect. This study was therefore designed to investigate the role of high salt-diet on reproductive and biochemical indiceses and the possible effect of the common plants mixture that is being used; which is a mixture of Annona murikata .L, Aloe barbadensis miller, Carica papaya .L, Moringa olivera .L.
Extraction from the plants used in this study followed standard biochemical procedure and the animal used for this study were 90 male wister rats grouped to nine (9) sections (A-I) based on the feeding and administration designs, group A, B and C received normal rats chow, high salt diet and high salt diet with standard drug respectively, groups DEF were given high salt diet with 100mg/kg, 200mg/kg, 300mg/kg respectively. While 100mg/kg, 200mg/kg and 300mg/kg of extract were administered to rats in groups GH and I respectively after being fed normal rats chow. The experiment lasted for 28 days, Animals were sacrificed on the 29th day after 12 hours of over night fasting and their blood sample and testis were removed for biochemical analysis of lipid profile in the serum, enzymatic activities of (G6PD, ALP and LDH) in the serum and testis homogenase, hormonal profile (testosterone, FSH and LH) and serum electrolyte concentrations of Sodium and Potassium were also assayed. Histology of testis was done using H and E staining, GCMS analysis was used to check the possible bioactive compounds presents in the mixture and SPSS version 19 software was used for the statistical analysis.
The result showed the presence of 71 bioactive compounds with benzene-2-(tertbuty-dimetysily)-oxyl-1-180propy-4-metyl- and deoxiguinghausu at peak % of 8.83 and 8.46 respectively. The biochemical analysis showed a significant reduction (P<0.05) in the levels.
Audience Take Away:
The extract is non toxic, easy to prepare, and possess great curing effect and the plants are easily assessable.
- The listeners will learn the correct formula combination for these medicinal plants in the treatment of diseases associated with male reproductive system.
- Also the bioactive compounds identified in this work could be subjected to further studies to investigate their bioactivity as novel candidates for drug development especially in the treatment of malaria.
- It was observed in this study that the medicinal plant used for the mixture if well combined in the correct proportion could inhibit the potential toxins present in the extract of the individual plant separately.
- Finally this extract was found to have a great corrective effect on the lipid profile which has been implicated in the pathogenesis of several other diseases.
