Title : Determination of total phenolic, total flavonoid, total alkaloids, total tannin, total aldehyde and antioxidant activity of crude drug
Abstract:
The evaluation of phytochemicals and antioxidant activity in crude drugs is essential for understanding their therapeutic potential and guiding drug development. This study aims to quantify total phenolic content, total flavonoids, total alkaloids, total tannins, and total aldehydes in crude drug samples, and assess their antioxidant activities. Various crude drug samples were subjected to chemical analysis using standard methods such as the Folin-Ciocalteu method for phenolics, aluminum chloride colorimetric assay for flavonoids, bromocresol green spectrophotometric method for alkaloids, vanillin-HCl method for tannins, and 2,4-DNPH method for aldehydes. The antioxidant activity was evaluated using the DPPH radical scavenging assay and the ABTS assay. Results indicated a wide range of phytochemical concentrations among the samples. Phenolic content ranged from X to Y mg GAE/g, flavonoids from A to B mg QE/g, alkaloids from M to N mg AE/g, tannins from C to D mg TAE/g, and aldehydes from E to F mg/g. The antioxidant activity showed a strong correlation with the total phenolic and flavonoid content, suggesting that these compounds significantly contribute to the antioxidant potential of the crude drugs. This study provides valuable insights into the phytochemical composition and antioxidant properties of crude drugs, emphasizing their potential use in the development of natural therapeutic agents. The findings highlight the importance of phytochemicals in crude drugs and their possible applications in health care, promoting further research into their mechanisms of action and therapeutic uses.
