Janaki Baral

Title : Antidiabetic and antioxidant properties of sarcococca coriacea hook and sarcococca wallichii staph of Nepali origin

Abstract:

Diabetes related complications has become a severe health problem globally. Lack of effective treatment and side effects due to synthetic drugs has led to increasing demand on plant based medications. Local value on ethno medicine and their herbal formulations to cure for diabetic and related complications are increasing mostly in places lagging easy access of medicine. Plants belonging to Sarcococca genus are medicinally important as locals claim the benefits of this species in curing diabetes. Thus this research focused on antidiabetic and antioxidant properties using ,  in vitro α-glucosidase, and α-amylase inhibition activity of methanolic extract of Sarcococca coriacea leaf ( Sc-A), Sarcococca coriacea stem (Sc-B), and dichloromethane fraction of methanolic extract of Sarcococca wallichii (Sw-D). Research revealed dichloromethane fraction of S. wallichii (Sw-D) with good inhibition of α- amylase enzyme (IC₅₀= 2.116 ± 0.058), whereas Sc-B inhibits α-glucosidase (20.97±2.37). Similarly, Sc-A showed significant antioxidant activity with IC₅₀=24.56±3.3. The total phenolic content on Sc-A and Sc-B was calculated as 151.35±4.42 mg GAE/g and 86.22±1.59 mg GAE/g whereas the total flavonoid content on Sc-A and Sc-B was found to be 21.61±4.88 mg QE/g and 24.09±4.02 mg QE/g respectively. Similarly, total phenolic and total flavonoid content on Sw-D were found to be 85.26±3.16 mg GAE/g and 21.57±1.26 mg QE/g. Very limited plants are claimed to possess activities on both these enzymes. Result proved potencies of these plant in both α-glucosidase and α-amylase inhibition activity. Thus this research scientifically supports use of these plants to manage diabetes by local people and has explored new plants for higher level of antidiabetic drug discovery.

Biography:

Mrs. Janaki Baral. She served as lecturer at Little Angels’ Higher Secondary School (2010-2017) Lalitpur after completing her masters. She is serving nation as an assistant Professor in one of the oldest and largest University of Nepal: Tribhuvan University, Nepal since 2017. Her MSc is in organic chemistry with interest on the research areas of isolation of compounds from natural products, in-vivo and in-vitro determination of antidiabetic property, toxicity determination, anti-cancerous activity determination of plant extracts, fractions and compounds, and other biological activity determination. She had completed her M.Phil. in Educational Leadership from Kathmandu University. She then joined the group of Associate Professor Dr. Achyut Adhikari for her PhD at Tribhuvan University. She was an ANRAP fellow (2018), at Bangladesh University of Health Sciences, Bangladesh. She had also visited University of Pakistan for her research activity. She is co-author of 2 articles, national and international. Active involvement in social activities.