Title : A Scientific approach to derive a Poly herbal Anti – inflammatory formula on the basis of some invitro -anti-Inflammatory, anti-arthritic activities by inhibition of protein denaturation method, COX enzyme inhibitory evaluation and with subsequent Phytoch
Abstract:
A methodology/protocol is tried out to evolve an herbal formula for purpose of treating inflammatory conditions as an alternative to NSAID’s. The brief out line of this analytical study is as mentioned as follows. a. Selection of herbs was done as per Classical Ayurvedic references, b. Authentified as per API (Ayurvedic Pharmacopeia of India), c. Extraction of each drug was done by different ratios of Hydroalcoholic menstrums, d. Invitro assesment of each extract after removing residual solvent, for anti-Inflammatory, anti-arthritic activities (by UV-Vis. Spectrophotometer with positive control), e. Invitro assessment of each extract for COX enzyme inhibition(by UV-Vis. Spectrophotometer with positive control), f. Selection of the extracts was made having good invitro activity, g. Animal studies (efficacy & toxicity) h. Performed the QC testing of each selected extract including HPTLC, that is the in process QC specifications, i. Decision of the single dose with mixtures of selected extracts was made as per the level of invitro activity and available toxicology data, j. Quantification of major groups like Phenolics, Flavonoids, Alkaloids and Bitters was done with both standard Spectrophotometric and Gravimetric methods, h. Method for Marker assay was developed and validated by HPTLC and a good resolved HPTLC finger print was developed for the single dosage API (Active Pharmaceutical Ingredient mixture of extracts), i. Three batches was prepared to fix the in process and API (Active Pharmaceutical Ingredient) QC specifications.
