Title : In-vivo comparative efficacy study of herbomineral formulation (hinguleshwar rasa) and indomethacin on FCA induced Rheumatoid arthritis in paw model of rats
Abstract:
As per Ayurveda symptoms of Rheumatoid Arthritis resembles Amavata disease. Hinguleshwar rasa is one of the Herbomineral formulations in Ayurveda, the action of Hinguleshwara rasa on Amavata was mentioned in the text of Rasatarangini in Ayurveda. In this study, an investigation was made to find out preventive & curative changes that occurred in rat paw of Freuds Complete Adjuvant induced RA model. Indomethacin was kept as Standard control and Hinguleshwar rasa as a test (Treatment) group. After induction of Arthritis with FCA on the day ‘14’, animals were treated up to the 28th day of the study with Indomethacin and Hinguleshwar rasa. In this study Anti-inflammatory activity of Hinguleshwar rasa was evaluated by measuring paw volume and after completion of the 28th day of daily single oral dose administration of Hinguleshwar rasa and Indomethacin, animals were sacrificed. Ankle joints were severed and processed for histological studies.
In the ‘28’ days study, Standard drug Indomethacin & Test drug Hinguleshwar rasa was administered orally from the day ‘15’ (After administration of FCA and development of Arthritis). After administration of a single oral dose of Hinguleshwar rasa & Indomethacin per day for the next ‘14’ days, it was observed that, swelling of paw volume of rats was significantly reduced in the Standard and Test group of animals on day 28th and also observed that the joints of Hinguleshwar rasa treated animals showed recovery towards normal histological architecture whereas all other joint samples of bones of rats were showing focal and minimal pathological changes, focal and minimal congestion of blood vascular tissue. A similar normal Histo-architecture was found in Indomethacin treated animals. However, it was observed that Hinguleshwar rasa showed better restoration of joint Histopathological changes that occurred due to FCA damage.
