In this research, 5 plant species in family Zingiberaceae were initially analysed for phytochemicals, total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant properties, as well as their anti-acetylcholinesterase inhibition. Alpinia nigra, Amomum biflorum, Amomum koenigii, Boesenbergia maxwellii and Etlingera araneosa were collected from Queen Sirikit Botanic Garden (QSBG) living collection in Thailand. The ethanol rhizome extracts gave percent yield ranged from 3.81-7.00 % (w/w). The results of anti-acetylcholinesterase inhibition of ginger plants are expressed with IC50 in ranged of 1.49- 4.94 mg/ mL. A. koenigii gave the best results of anti-oxidative property by 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay, anti-acetylcholinesterase, percent yield, TPC and TFC. While A. biflorum showed the best antioxidant value with IC50 1.63 ± 0.02 by 2,2-diphenyl-1-picrylhydrazyl (DPPH). The chemical constituents of ethanol rhizome extracts were also characterised using High Performance Thin Layer Chromatography (HPTLC) and Thin Layer Chromatography/ Compact Mass Spectrometer (TLC/ CMS), while the quantitative analyses of their chemical compounds were analysed by HPLC compared to standards (i.e., gallic acid, caffeic acid, vanillin, quercetin, luteolin and cyanidin chloride). The most active extracts showed the presence of phenolic and flavonoid compounds, these characteristics may be related with strong anti-Alzheimer and antioxidants.