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Paris, France
September 01-02, 2022 | Paris, France
ICTM 2019

The extracts from wild bitter gourd fruit reduced the expression of inflammatory cytokines in TNF treated pulmonary epithelial cells via the PI3K AKT NF KB pathway

Yuh Lien Chen, Speaker at Global Event on Ethnomedicine
National Taiwan University, Taiwan
Title : The extracts from wild bitter gourd fruit reduced the expression of inflammatory cytokines in TNF treated pulmonary epithelial cells via the PI3K AKT NF KB pathway

Abstract:

Wild bitter gourds (WBG, Momordica charantia L.) is eaten as a vegetable and used as a traditional herb in Asia. The significant pharmacological properties of WBG fruit extract (WBGE) are anti-diabetic, antiinflammatory, anti-tumor and anti-oxidant actions. However, the anti-inflammatory effects of WBGE on human lung epithelial cells and their underlying mechanisms require further investigation. To analyze the effect of WBGE and charantin on ICAM-1 and IL-6 expression under inflammation, pre-incubated A549 cells with WBGE for 24 h, 10, 30, 50, or 80 μg/mL or charantin, 0.5, 1, 3, 5 μg/mL followed by 3 ng/mL TNF-α was stimulated for 4 h. TNF-α treatment significantly increased the expression of ICAM-1 and IL-6 in A549 cells. Pretreatment with WBGE or charantin or aspirin (positive control) inhibited this effect. WBGE significantly reduced the expression of inflammatory cytokines in a concentration-dependent manner. Consistently, fluorescence microscopy images showed that ICAM-1 and IL-6 were strongly present in the cytosol of TNFα-treated A549 cells. In contrast, ICAM-1 expression was weaker in TNF-α-stimulated A549 cells pretreated with WBGE or charantin. Inhibition of PI3K / AKT phosphorylation mediates the reduction of ICAM-1 and IL-6 expression by WBGE in TNF-α-treated A549 cells. Inhibition of NF-κB p65 activation and translocation mediates the expression of ICAM-1 and IL-6 in WBGE-reduced TNF-α-treated A549 cells. WBGE reduced ICAM-1 and IL-6 expression in lung tissue of TNF-α treated WT mice. These results indicate that WBGE reduces the expression of inflammatory cytokines in both in vitro and in vivo conditions. The protective part is mediated through the PI3K / AKT / NF-κB pathway

Biography:

Dr. Yuh-Lien Chen studied Anatomy and Cell Biology at the College of Medicine, National Taiwan University and graduated as PhD in 1990. The major field of her study was to investigate the pathogenesis of cardiovascular diseases and inflammation and their related mechanisms. She obtained the position of a Professor in 2004. She has published more than 100 research articles in SCI journals.

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