Title : The effects of Brazilian propolis and contained flavonoids on platelet aggregation and blood coagulation system
Propolis has been used worldwide as a dietary supplement for the purpose of health promotion. Recently, some studies have shown that a diet in fruit and vegetables containing flavonoid reduced the risk of thrombosis and cardiovascular disease. We hypothesized Brazilian propolis (AF-08) contained some flavonoids has antithrombotic action. We determined the effects of AF-08 and three flavonoids (apigenin, kaempferol and chrysin) contained in AF08 against blood coagulation system and platelet aggregation. Plasma from healthy volunteers (The study protocol was approved by our institutional ethics committees.) were incubated with serial-diluted AF-08, apigenin, kaempferol, or chrysin for 10 minutes. The effects of AF-08, apigenin, kaempferol and chrysin on blood coagulation system and platelet function were evaluated by prothrombin time (PT), activated partial thromboplastin time (APTT) and platelet aggregation, respectively. Consequently, AF-08, apigenin, and chrysin significantly inhibited collagen-induced platelet aggregation in human plasma. However, they did not affect PT and APTT. Kaempferol did not affect in all measured parameters. These results suggest that AF-08, apigenin, and chrysin inhibit platelet aggregation without affecting blood coagulation system. Therefore, AF-08, apigenin, and chrysin might have potential to suppress platelet-based arterial thrombosis.