Title : Anti-HIV-1 protease activity of the crude extracts and isolated compounds from Auricularia polytricha
Abstract:
Acquired immunodeficiency syndrome (AIDS) is caused by the Human immunodeficiency virus type-1 (HIV1). HIV-1 protease (PR) is an essential enzyme for the HIV replication. Auricularia polytricha (AP) is an edible mushroom that has several important therapeutic properties. The sequential hexane (APH), ethanol (APE) and water (APW) extracts from AP were screened for inhibitory activity against HIV-1 PR. APH exhibited significant inhibition on HIV-1 PR activity. Phytochemical constituents of APH were purified by chromatography and structurally elucidated by 1D and 2D NMR, HRMS, FTIR, and GC/MS techniques. The isolated compounds were identified to be two triacylglycerols, linoleic acid and ergosterol. All isolated compounds showed significant inhibition of HIV-1 PR activity. The findings from this study suggest that AP is a good source of fatty esters, fatty acids and ergosterol which exhibit antiHIV-1 properties by blocking HIV-1 PR. These important biological results warrant further development of AP as an alternative antiretroviral drug
